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permeabilization buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher permeabilization buffer
    Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/permeabilization+buffer/Triton+X-100/pm42332882-110-12-19
    Average 99 stars, based on 1 article reviews
    permeabilization buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Longitudinal in vivo imaging of dynamic immune-vascular responses to meningeal cerebrovascular injury.
    Article Snippet: Vibratome sections underwent EdU staining following the manufacturer's instructions (Click-iT EdU; Invitrogen, cat# C10340). .. In short, tissue sections were permeabilized for 20-minute incubation at room temperature in a permeabilization buffer (1% Bovine Serum Albumin (Thermo Fischer CAT #B14) and 0.5% Triton X-100 (Promega CAT #H5142) in PBS). ..

    Article Title: Longitudinal in vivo imaging of dynamic immune–vascular responses to meningeal cerebrovascular injury
    Article Snippet: Vibratome sections underwent EdU staining following the manufacturer’s instructions (Click-iT EdU; Invitrogen, cat# C10340 ). .. In short, tissue sections were permeabilized for 20-minute incubation at room temperature in a permeabilization buffer (1% Bovine Serum Albumin (Thermo Fischer CAT #B14) and 0.5% Triton X-100 (Promega CAT #H5142) in PBS). ..

    Article Title: CGM23 corresponds to a pan-coronavirus lipopeptide inhibitor potently inhibiting virion fusion
    Article Snippet: The harvested mouse lungs were cut into 15 μm thick frozen sections with a cryostat (Leica CM 1900). and mounted to super frosted microscopic slides (VWR, 48311-703). .. The tissue sections were first washed in PBS and incubated in a blocking and permeabilization buffer [DPBS supplemented with 0.2 % Triton X-100, 3 % normal goat serum (Gibco, 16210074) and 5 % BSA] for 1 hour at room temperature. .. Sections were rinsed twice with PBS containing 0.1 % Triton X-100 before overnight incubation with anti-SARS-CoV-2 Spike rabbit polyclonal antibody (GeneTex, GTX135360) and anti-mouse MAC-2 monoclonal antibody (Cedarlane Labs, CL8942AP) (1:100 dilution) at 4 °C.

    Article Title: Longitudinal in vivo imaging of dynamic immune-vascular responses to meningeal cerebrovascular injury.
    Article Snippet: .. Afterwards, samples were further permeabilized with a 10- minute incubation in a permeabilization buffer (0.1% Triton X-100 and 1% sodium citrate (Thermo Fischer CAT #J63888.AK) in PBS). ..

    Article Title: Longitudinal in vivo imaging of dynamic immune–vascular responses to meningeal cerebrovascular injury
    Article Snippet: .. Afterwards, samples were further permeabilized with a 10-minute incubation in a permeabilization buffer (0.1% Triton X-100 and 1% sodium citrate (Thermo Fischer CAT #J63888.AK) in PBS). .. Sections were washed twice for 5 min with PBST and stained with TUNEL using kit instructions (TUNEL Assay Apoptosis Detection Kit, Biotium CAT #30074).

    Article Title: Mammarenaviruses depend on endogenous fatty acid synthesis in cell culture systems
    Article Snippet: .. VeroS cells were transfected via JetOptimus with pcDNA-LASV-Z-FLAG, pcDNA-LASV-Z-FLAG-G2Aor pcDNA-LCMV-Z-HA-YFP for 5 h before transfection inoculum was removed and cells were treated with DMSO (0.1%v/v), TVB-2640 (33 μM), or IMP-1088 (1 μM) for 24 h. Cells were fixed in ice-cold formalin (3.7%v/v) for 20 min, quenched in 50 mM glycine in PBS for 30 min, and with the exception of pcDNA-LCMV-Z-HA-YFP, permeabilized in permeabilization buffer (0.1%v/v Triton X-100, 2%w/v BSA, 0.02%w/v sodium azide, PBS) at RT for 20 min. After washing in PBS, cells were blocked in blocking media (10%w/v BSA, 0.02%w/v sodium azide, PBS) for 1 h, followed by incubation in FLAG staining buffer (1:1,000X anti-FLAG-AF647 (Invitrogen cat#701629RP647), 2%w/v BSA, 0.02%w/v sodium azide, PBS) overnight. ..

    Concentration Assay:

    Article Title: Protocol for immunofluorescence detection and quantitative analysis of pH-dependent transcriptional condensates.
    Article Snippet: .. Permeabilization buffer (0.5% Triton X-100 in PBS) Reagent Final concentration Amount Triton X-100 0.5% 250 μL PBS without Ca 2+ and Mg 2+ Gibco, #10010-023 99.5% 49.75 mL Total N/A 50 mL Mix thoroughly until Triton X-100 is fully dissolved. ..

    Labeling:

    Article Title: VEGFR2 dimerization kinetics on the cell surface determined by microscopy and mathematical modeling.
    Article Snippet: Inter-receptor interactions play a key role in cell signaling in response to external stimuli.. For quantitative understanding and modeling of transmembrane signal transduction, it is necessary to quantify receptor interaction kinetics (i.e., association and dissociation rate constants) on the cell surface.. Live-cell singlemolecule imaging (SMI) has the powerful ability to capture transient receptor interaction events in their native cellular environment with high spatiotemporal resolution.

    Blocking Assay:

    Article Title: CGM23 corresponds to a pan-coronavirus lipopeptide inhibitor potently inhibiting virion fusion
    Article Snippet: The harvested mouse lungs were cut into 15 μm thick frozen sections with a cryostat (Leica CM 1900). and mounted to super frosted microscopic slides (VWR, 48311-703). .. The tissue sections were first washed in PBS and incubated in a blocking and permeabilization buffer [DPBS supplemented with 0.2 % Triton X-100, 3 % normal goat serum (Gibco, 16210074) and 5 % BSA] for 1 hour at room temperature. .. Sections were rinsed twice with PBS containing 0.1 % Triton X-100 before overnight incubation with anti-SARS-CoV-2 Spike rabbit polyclonal antibody (GeneTex, GTX135360) and anti-mouse MAC-2 monoclonal antibody (Cedarlane Labs, CL8942AP) (1:100 dilution) at 4 °C.

    Article Title: Mammarenaviruses depend on endogenous fatty acid synthesis in cell culture systems
    Article Snippet: .. VeroS cells were transfected via JetOptimus with pcDNA-LASV-Z-FLAG, pcDNA-LASV-Z-FLAG-G2Aor pcDNA-LCMV-Z-HA-YFP for 5 h before transfection inoculum was removed and cells were treated with DMSO (0.1%v/v), TVB-2640 (33 μM), or IMP-1088 (1 μM) for 24 h. Cells were fixed in ice-cold formalin (3.7%v/v) for 20 min, quenched in 50 mM glycine in PBS for 30 min, and with the exception of pcDNA-LCMV-Z-HA-YFP, permeabilized in permeabilization buffer (0.1%v/v Triton X-100, 2%w/v BSA, 0.02%w/v sodium azide, PBS) at RT for 20 min. After washing in PBS, cells were blocked in blocking media (10%w/v BSA, 0.02%w/v sodium azide, PBS) for 1 h, followed by incubation in FLAG staining buffer (1:1,000X anti-FLAG-AF647 (Invitrogen cat#701629RP647), 2%w/v BSA, 0.02%w/v sodium azide, PBS) overnight. ..

    Transfection:

    Article Title: Mammarenaviruses depend on endogenous fatty acid synthesis in cell culture systems
    Article Snippet: .. VeroS cells were transfected via JetOptimus with pcDNA-LASV-Z-FLAG, pcDNA-LASV-Z-FLAG-G2Aor pcDNA-LCMV-Z-HA-YFP for 5 h before transfection inoculum was removed and cells were treated with DMSO (0.1%v/v), TVB-2640 (33 μM), or IMP-1088 (1 μM) for 24 h. Cells were fixed in ice-cold formalin (3.7%v/v) for 20 min, quenched in 50 mM glycine in PBS for 30 min, and with the exception of pcDNA-LCMV-Z-HA-YFP, permeabilized in permeabilization buffer (0.1%v/v Triton X-100, 2%w/v BSA, 0.02%w/v sodium azide, PBS) at RT for 20 min. After washing in PBS, cells were blocked in blocking media (10%w/v BSA, 0.02%w/v sodium azide, PBS) for 1 h, followed by incubation in FLAG staining buffer (1:1,000X anti-FLAG-AF647 (Invitrogen cat#701629RP647), 2%w/v BSA, 0.02%w/v sodium azide, PBS) overnight. ..

    Staining:

    Article Title: Mammarenaviruses depend on endogenous fatty acid synthesis in cell culture systems
    Article Snippet: .. VeroS cells were transfected via JetOptimus with pcDNA-LASV-Z-FLAG, pcDNA-LASV-Z-FLAG-G2Aor pcDNA-LCMV-Z-HA-YFP for 5 h before transfection inoculum was removed and cells were treated with DMSO (0.1%v/v), TVB-2640 (33 μM), or IMP-1088 (1 μM) for 24 h. Cells were fixed in ice-cold formalin (3.7%v/v) for 20 min, quenched in 50 mM glycine in PBS for 30 min, and with the exception of pcDNA-LCMV-Z-HA-YFP, permeabilized in permeabilization buffer (0.1%v/v Triton X-100, 2%w/v BSA, 0.02%w/v sodium azide, PBS) at RT for 20 min. After washing in PBS, cells were blocked in blocking media (10%w/v BSA, 0.02%w/v sodium azide, PBS) for 1 h, followed by incubation in FLAG staining buffer (1:1,000X anti-FLAG-AF647 (Invitrogen cat#701629RP647), 2%w/v BSA, 0.02%w/v sodium azide, PBS) overnight. ..



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